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Early Postnatal NT‐3 Gene Delivery Enhances Hearing Acquisition in the Developmental Period
Author(s) -
Jin Yongde,
Lyu AhRa,
Park SungJae,
Xu Jun,
Cui Jie,
Sohn KyungCheol,
Hur Gang Min,
Jin Yulian,
Park YongHo
Publication year - 2016
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.26130
Subject(s) - spiral ganglion , neurotrophin 3 , cochlea , green fluorescent protein , round window , biology , auditory brainstem response , viral vector , andrology , medicine , hearing loss , receptor , anatomy , audiology , gene , neurotrophic factors , recombinant dna , brain derived neurotrophic factor , biochemistry
Objectives/Hypothesis The purpose of this study was to investigate the effect of early postnatal neurotrophin‐3 (NT3) support on hearing acquisition. Study Design A prospective experimental animal study. Methods Adenoviral (Ad) vectors expressing green fluorescence protein (GFP) alone or in combination with NT3 were injected into the scala tympani through the round window of 5‐postnatal‐day‐old (P5) rats. Changes in NT3 mRNA level, hearing thresholds, and morphological studies were done after the viral vector injection. Results NT3 mRNA was significantly increased in the Ad‐GFP‐NT3 group compared to the normal‐developmental group and Ad‐GFP alone group. GFP was widely expressed in the cochlea such as in the hair cells, supporting cell area, and spiral ganglion neurons. Auditory brainstem response thresholds were significantly lower in the Ad‐GFP‐NT3 group compared to the normal‐developmental group and Ad‐GFP alone group at P15. Conclusions These results show that early postnatal NT3 overexpression may accelerate the acquisition of hearing in rats. Level of Evidence NA Laryngoscope , 126:E379–E385, 2016