Premium
Prevention of anterior glottis web with hyaluronic acid derivatives in rabbit model
Author(s) -
Kwon Seong Keun,
Ryu YoonJong,
Kim Dong Wook,
Chang Hyun,
Kim Soo Yeon,
Sung Myung Whun,
Hah J. Hun
Publication year - 2016
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.26089
Subject(s) - medicine , glottis , new zealand white rabbit , vocal folds , saline , surgery , larynx , biology , paleontology
Objectives/Hypothesis Validating an antiadhesive strategy to prevent anterior glottic web (AGW) development. Study Design Prospective animal study in rabbits. Methods Fifteen New Zealand white rabbits were randomly divided into three equal groups (five rabbits each), applying either HyFence (HF: 0.1 g/vocal fold), an antiadhesive test agent; mitomycin‐C (MMC: 1 mg/ml, 4 min) as reference therapy; or saline (control group) to AGWs induced by laryngofissure technique (previously reported by us). At week 8, the extent of AGW was measured to assess preventive effects of HF and MMC, and laryngeal specimens were sampled for high‐speed recording and histologic analysis. Results AGW induction was successful in all five (100%) control rabbits, each demonstrating fibroconnective tissue and collagen fibers at anterior commissure, with AGW ratio averaging 0.57 ± 0.052. An AGW was seen in 100% of the rabbits survived in the MMC group (n = 2) with the 60% (n = 3) of the MMC rabbits expired due to postprocedure infections. The average AGW ratio was 0.28 ± 0.070 in the MMC group. AGW formation rate was 50% in HF group with one rabbit expired from infection. Mean AGW ratio was lowest in the HF group (0.23 ± 0.10; P = 0.013). Microscopically, AGW induction sites in HF‐treated rabbits displayed significantly fewer collagen fibers ( P = 0.009) by comparison. Conclusion This is the first reported use of a cross‐linked hyaluronic acid gel that may prevent AGW formation. Level of Evidence N/A. Laryngoscope , 126:2320–2324, 2016