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Effects of peritumoral nanoconjugated cisplatin on laryngeal cancer stem cells
Author(s) -
Sim Michael W.,
Grogan Patrick T.,
Subramanian Chitra,
Bradford Carol R.,
Carey Thomas E.,
Forrest M. Laird,
Prince Mark E.,
Cohen Mark S.
Publication year - 2016
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.25808
Subject(s) - cisplatin , cd44 , medicine , cancer , ex vivo , in vivo , cancer stem cell , pathology , cancer research , oncology , chemotherapy , cell , biology , genetics , microbiology and biotechnology
Objectives/Hypothesis To evaluate the efficacy of peritumoral hyaluronic acid (HA)‐cisplatin therapy in a murine model of laryngeal squamous cell carcinoma and to evaluate its effect on cancer stem cells (CSCs). Study Design An orthotopic murine study utilizing University of Michigan squamous cell carcinoma‐12 (UMSCC‐12) laryngeal cancer cells was conducted in randomized controlled fashion with three treatment arms: saline, systemic cisplatin, and peritumoral HA‐cisplatin. Methods UMSCC‐12 laryngeal cancer cells were inoculated into the buccal mucosa of athymic nude mice followed by weekly treatment with saline, systemic cisplatin, or peritumoral HA‐cisplatin for 3 weeks. Tumor response and animal weight was monitored and change in CD44 proportion was analyzed ex vivo. Results HA‐cisplatin demonstrated superior antitumor efficacy and greater reduction in CD44 positivity on ex vivo analysis. Conclusions Peritumoral nanoconjugated HA‐cisplatin provides superior antitumor efficacy compared to standard cisplatin therapy in an in vivo laryngeal cancer model. There was also selective targeting of CD44+ cancer cells with HA‐cisplatin. This therapeutic strategy could represent the first selective laryngeal CSC‐targeted therapy. Further preclinical investigation is warranted to evaluate its role for locally advanced head and neck cancer treatment. Level of Evidence NA Laryngoscope , 126:E184–E190, 2016