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Upregulation of Caveolin‐1 correlate with A kt expression and poor prognosis in NPC patients
Author(s) -
Wang YingPiao,
Lin ChihFeng,
Tsai ShuChun,
Tsai ChingHwa,
Yeh TeHuei
Publication year - 2015
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.25297
Subject(s) - small hairpin rna , gene knockdown , protein kinase b , transfection , blot , nasopharyngeal carcinoma , cancer research , immunohistochemistry , cell culture , biology , rna interference , cell growth , messenger rna , downregulation and upregulation , microbiology and biotechnology , signal transduction , medicine , immunology , gene , rna , biochemistry , genetics , radiation therapy
Objectives The aim of this study is to investigate the role of Caveolin‐1 (Cav‐1) in nasopharyngeal carcinoma (NPC) progression and correlated with clinical outcomes. Methods We used quantitative real‐time PCR (qPCR) to detect the difference in the expression of mRNA level of Cav‐1 mRNA in NPC, non‐NPC cell lines, and 74 NPC and 29 nontumorous nasopharyngeal mucosa biopsies. Western blotting and immunohistochemistry staining were used to detect the protein expression of Cav‐1 in cell lines and biopsy tissues. We collected clinical follow‐up data to investigate the association with expression of Cav‐1 mRNA. Also, transfection of Cav‐1 and suppression by delivery of shRNA against Cav‐1 into NPC derived cell lines to analyze its influence in Akt signaling. Results By use of qPCR, immunohistochemical staining, and western blotting, we found that not only is Cav‐1 overexpressed in human NPC tumor cells and NPC‐derived cell lines but high Cav‐1 mRNA expression is associated with poor overall survival time of NPC patients. Furthermore, phosphorylated Akt expression was enhanced by Cav‐1 transfection and suppressed by delivery of shRNA against Cav‐1. These data suggested a possible regulatory mechanism of Cav‐1 on Akt signaling pathway. We also transfected the Cav‐1 construct and shRNA in TW01 cells to prove the effect on Akt protein expression. Conclusions Overexpression of Cav‐1 is related to poor prognosis in NPC patients, which correlated with Akt signaling pathway. Abrogation of Akt signaling by shRNA‐mediated knockdown of Cav‐1 decreased malignant properties of tumor cells. These data suggest the potential for Cav‐1 as a possible novel therapeutic target in NPC treatment. Level of Evidence N/A. Laryngoscope , 125:E231–E238, 2015