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Modulation of satellite cells activity and M yo D in rat thyroarytenoid muscle after reinnervation
Author(s) -
Kodama Haruka,
Kumai Yoshihiko,
Nishimoto Kohei,
Sanuki Tetsuji,
Yumoto Eiji
Publication year - 2015
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.25248
Subject(s) - reinnervation , myod , denervation , medicine , myogenic regulatory factors , myogenin , cadherin , anatomy , endocrinology , biology , myocyte , myogenesis , cell , genetics
Objectives/Hypothesis To examine modulation of M‐cadherin, a marker for satellite cells (SCs); and MyoD, which may indicate the myogenic activity following recurrent laryngeal nerve (RLN) denervation and immediate reinnervation; and to elucidate the correlation between their modulations and establishment of neuromuscular junctions (NMJs) in the reinnervated rat thyroarytenoid (TA) muscle. Study Design Quantitative real‐time polymerase chain reaction qPCR and histologic assessment of the TA muscle following RLN transection and anastomosis. Methods Rats were divided into three groups: 1) denervation alone (DNV) (n = 60), 2) denervation with anastomosis (ANS) (n = 60), and 3) sham‐operated controls (n = 12). Animals were sacrificed at 3 days and 1, 3, and 5 weeks after treatment. TA muscles harvested from 40 animals from each DNV and ANS group; all of sham group were subjected to qPCR for assessment of the modulation of M‐cadherin and MyoD; and the remaining larynges of DNV and ANS group were used for histologic analysis. Results The expression levels of messenger RNAs (mRNAs) encoding M‐cadherin and MyoD in the TA muscle of the DNV group were significantly higher ( P < 0.05) than in the control throughout the study period. These mRNA levels in the ANS group were significantly higher ( P < 0.05) at ≤ 1 week than in the controls but fell to control levels at ≥ 3 weeks. In the ANS group, recovery of muscle area and NMJs structure occurred by 3 weeks. Conclusion These data suggested that NMJ formation following reinnervation might prompt recovery of M‐cadherin and MyoD mRNA expression to the quiescent level of SCs. Level of Evidence N/A. Laryngoscope , 125:E245–E251, 2015