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Effects of a continuous electromagnetic field on wound healing in human airway
Author(s) -
Kim DongHyun,
Kim Hyun Jun,
Gimm YoonMyoung,
Hong Sung Pyo,
Jeon Eunju,
Park Eun Young
Publication year - 2015
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.25109
Subject(s) - wound healing , airway , electromagnetic field , field (mathematics) , medicine , anesthesia , physics , surgery , mathematics , quantum mechanics , pure mathematics
Objectives/Hypothesis The aim of this study was to investigate the effect of a 1.8‐GHz continuous electromagnetic field (EMF) on wound healing in a human airway cell‐culture system. Study Design In vitro study using a cell line. Methods Immortalized human bronchial epithelial cells (a BEAS‐2B cell line) were exposed to a 1.8‐GHz EMF (specific absorption rate = 1.0 W/kg). We evaluated the effect of EMF on the cells using an 3‐(4,5‐dimethylthiazol‐2yl)‐2,5‐diphenyltetrazolium bromide (MTT) viability assay, by cell counting, and by using fluorescence‐activated cell sorting (FACS) analysis of cell cycle dynamics and apoptosis. Inhibition of migration was tested by a wound‐healing assay on scratched cell cultures. Results Cell migration in the wound‐healing assay was decreased by the EMF treatment compared with controls. The MTT assay and cell counting consistently showed that the EMF used was not cytotoxic and did not inhibit cell proliferation. FACS analysis showed no alterations in the cell‐cycle phase distribution or in apoptosis after EMF exposure. Conclusion EMF can inhibit wound healing in vitro by inhibiting cell migration. Level of Evidence N/A. Laryngoscope , 125:1588–1594, 2015