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An animal model for laryngotracheal injuries: An experimental study
Author(s) -
Kumar Saravanam P.,
Ravikumar Arunchalam,
Thanka J.
Publication year - 2015
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.24867
Subject(s) - medicine , intubation , endotracheal intubation , larynx , respiratory distress , histopathological examination , animal model , anesthesia , surgery , laryngoscopy , animal study , airway , pathology
Objectives/Hypothesis To establish an animal model for post‐intubation laryngotracheal injuries resulting in significant laryngotracheal insult without compromising the survival of the animal for a minimum period of 12 weeks post‐extubation. To study the extent of injury based on morphometric and histopathological change seen at 12 weeks post‐extubation. Study Design Randomized block, single‐blinded, experimental study. Methods Twelve New Zealand white rabbits were used in this study. The rabbits were randomly divided into three groups depending on the duration of intubation (6 hours, 4 hours, and 2 hours, respectively). The technique of intubation was standardized. Post‐extubation, these rabbits were humanely sacrificed after observation for 12 weeks or when they developed severe respiratory distress. The larynx and trachea were subjected to gross and histopathological examination. Results Histopathological examination and statistical analysis showed significant subglottic injury in all group B rabbits (intubated for 4 hours), and all of them survived for 12 weeks. Conclusion In this study, an animal model for inducing and studying laryngotracheal injuries due to endotracheal intubation has been established. This study has the potential applications in research on etiopathogenesis and management of post‐intubation laryngotracheal injuries. Level of Evidence N/A. Laryngoscope , 125:E23–E27, 2015