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Effects of phonation time and magnitude dose on vocal fold epithelial genes, barrier integrity, and function
Author(s) -
Kojima Tsuyoshi,
Valenzuela Carla V.,
Novaleski Carolyn K.,
Van Deusen Mark,
Mitchell Joshua R.,
Garrett C. Gaelyn,
Sivasankar M. Preeti,
Rousseau Bernard
Publication year - 2014
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.24827
Subject(s) - phonation , intensity (physics) , medicine , desquamation , occludin , gene expression , pathology , biology , tight junction , gene , microbiology and biotechnology , audiology , physics , quantum mechanics , biochemistry
Objectives/Hypothesis To investigate the effects of increasing time and magnitude doses of vibration exposure on transcription of the vocal fold's junctional proteins, structural alterations, and functional tissue outcomes. Study Design Animal study. Methods 100 New Zealand White breeder rabbits were studied. Dependent variables were measured in response to increasing time doses (30, 60, or 120 minutes) and magnitude doses (control, modal intensity, and raised intensity) of vibration exposure. Messenger RNA expression of occludin, zonula occluden‐1 (ZO‐1), E‐cadherin, β‐catenin, interleukin 1β, cyclooxygenase‐2, transforming growth factor β‐1, and fibronectin were measured. Tissue structural alterations were assessed using transmission electron microscopy (TEM). Transepithelial resistance was used to measure functional tissue outcomes. Results Occludin gene expression was downregulated in vocal folds exposed to 120‐minute time doses of raised‐intensity phonation, relative to control, and modal‐intensity phonation. ZO‐1 gene expression was upregulated following a 120‐minute time dose of modal‐intensity phonation, compared to control, and downregulated after a 120‐minute time dose of raised‐intensity phonation, compared to modal‐intensity phonation. E‐cadherin gene expression was downregulated after a 120‐minute time dose of raised‐intensity phonation, compared to control and modal‐intensity phonation. TEM revealed extensive desquamation of the stratified squamous epithelial cells with increasing time and magnitude doses of vibration exposure. A general observation of lower transepithelial resistance measures was made in tissues exposed to raised‐intensity phonation compared to all other groups. Conclusions This study provides evidence of vocal fold tissue responses to varying time and magnitude doses of vibration exposure. Level of Evidence NA Laryngoscope , 124:2770–2778, 2014