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Mesenchymal stem cells and nanofibers as scaffolds for the regeneration of thyroid cartilage
Author(s) -
Jotz Geraldo P.,
Luz Soster Paula R.,
Kunrath Seno O.,
Steffens Daniela,
Braghirolli Daikelly I.,
Zettler Claudio Galleano,
Beck Carlos A.,
Muccillo Marcelo,
Lopes Rui F. F.,
Mastella Bernardo,
Pranke Patricia
Publication year - 2014
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.24805
Subject(s) - cartilage , thyroid cartilage , mesenchymal stem cell , regeneration (biology) , chondrogenesis , scaffold , lesion , nanofiber , thyroid , anatomy , stem cell , chemistry , larynx , pathology , medicine , biology , biomedical engineering , materials science , microbiology and biotechnology , nanotechnology
Objectives/Hypothesis The aim of this study has been to establish an alternative approach in the form of regeneration of the thyroid cartilage. Study Design Four 1‐month old pigs (Sus scrofa) were used (divided into 3 groups) and submitted to general anesthetic to perform cervictomy with exposure of the thyroid cartilage in a total of 12 (twelve) samples. Method A resection of 4.0 cm 2 of cartilage was carried out in the right upper region and in the left upper and lower left region of the cartilage, where a scaffold with or without stem cells was implanted. In the left lower region, no biomaterial was implanted and the defect was left open (lesion control [L]). Results The average extension of the cartilaginous neoformation of L group was 136.3 μm (± 9.6) and 387.7 μm (± 43.2) in the scaffold (SCA) group, presenting a significant statistical difference ( P < 0.01). The analysis carried out on the lesion site sections of the cartilage of the larynx of the animals from the SCA group + mesenchymal stem cells (SCA+MSC) showed an average of the extension of neocartilage of 825.4 μm (± 122.1), showing a more extensive area of neocartilage when compared to the other groups. These results demonstrated a high significantly statistical difference ( P < 0.001) when compared with the L and SCA groups. Conclusion In 100% of the cases for which SCA+MSCs were used, a significant success in the cartilage growth and closing of the lesion in the thyroid cartilage was obtained compared to the other two groups for which MSCs were not used. Level of Evidence N/A. Laryngoscope , 124:E455–E460, 2014