Influence of continuous negative pressure in the rat middle ear

Objectives/Hypothesis High negative pressure in the middle ear was thought to be closely related to the etiology of retraction‐type cholesteatoma. Recently, it has been detected that mechanical forces are important factors in epithelial turnover and affect cytoskeletal remodeling. Continuous negative pressure in the middle ear may possibly accelerate the proliferation and differentiation of epithelial cells of the tympanic membrane. Study Design Animal experimental study. Methods Eleven adult male Sprague‐Dawley rats were used, and continuous negative pressure was loaded by connecting a catheter from the rat's middle ear to the supply route of an implantable microinfusion pump, iPRECIO. At 7 days after implantation of the device, an otoendoscopic examination and micro‐computed tomography (CT) analysis of the temporal bone were performed; the temporal bones were then collected for histological and immunohistochemical analysis. The degree of proliferation and differentiation of epithelial cells of the tympanic membrane was investigated immunohistochemically using the anti–cytokeratin‐5 and anti–cytokeratin‐10 antibodies. Results Otoendoscopic examination revealed retraction of the pars flaccida in all of the ears under negative pressure. In the micro‐CT analysis, soft tissue density area in the hypotympanum was observed in all ears under negative pressure. Histological analysis revealed thickened epithelium of the pars flaccida. In this region, the thickness of layers with cytokeratin‐5–positive cells and cytokeratin‐10–positive cells were increased. Conclusions Continuous negative pressure in the middle ear can lead to thickening of the epithelium of the pars flaccida, and may accelerate the proliferation and differentiation of epithelial cells. Level of Evidence NA Laryngoscope 124:2404–2410, 2014