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Friend or foe? Effect of oral resveratrol on cisplatin ototoxicity
Author(s) -
Olgun Yüksel,
Kırkım Günay,
Kolatan Efsun,
Kıray Müge,
Bagrıyanık Alper,
Olgun Aybüke,
Kızmazoglu Deniz Cakır,
Ellıdokuz Hülya,
Serbetcıoglu Bulent,
Altun Zekiye,
Aktas Safiye,
Yılmaz Osman,
Günerı Enis Alpin
Publication year - 2014
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.24323
Subject(s) - ototoxicity , cisplatin , tunel assay , auditory brainstem response , saline , resveratrol , medicine , cochlea , in vivo , pharmacology , anesthesia , hearing loss , biology , audiology , chemotherapy , immunohistochemistry , microbiology and biotechnology
Objectives/Hypothesis Our objectives were to study effects of orally administered resveratrol (RV) against cisplatin (CDDP) ototoxicity in different doses and to investigate ultrastructural changes in the cochlea and brainstem. Study Design In vivo study using an animal model. Methods Thirty‐two male Wistar albino rats were divided into six groups. Baseline distortion product otoacoustic emissions (DPOAEs) and auditory brainstem response (ABR) measurements were made. In groups I, II, and III, only saline, RV, and CDDP were given, respectively. Group IV, V, and VI animals were administered 10 mg/kg/day, 1 mg/kg/day, and 0.1 mg/kg/day of RV for 10 days, respectively, before 16 mg/kg CDDP injections were administered on day 11. All animals were sacrificed after repeated DPOAEs and ABR measurements were made on day 14. Cochleas of animals were investigated with transmission electron microscopy. Apoptosis were investigated with caspase‐3 activity and terminal deoxynucleotidyl transferase‐mediated dUTP‐biotin nick end labeling (TUNEL) method in the brainstem. Results In groups IV and V, DPOAEs and ABR findings revealed that oral administration of RV 10 mg/kg/day and 1 mg/kg/day doses before CDDP injection enhanced ototoxicity. In group VI, electomicroscopy revealed better ultrastructural findings than in the cisplatin group; however, these changes were not reflected in the audiological findings accordingly. Conclusions Our results implied that there were noticeable differences between different oral RV doses used for cisplatin ototoxicity. Especially in higher doses, RV was observed to enhance cisplatin ototoxicity. Level of Evidence N/A. Laryngoscope , 124:760–766, 2014