Premium
Cytokine correlation between sinus tissue and nasal secretions among chronic rhinosinusitis and controls
Author(s) -
Oyer Samuel L.,
Mulligan Jennifer K.,
Psaltis Alkis J.,
Henriquez Oswaldo A.,
Schlosser Rodney J.
Publication year - 2013
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.24305
Subject(s) - chronic rhinosinusitis , medicine , sinusitis , cytokine , sinus (botany) , immunology , correlation , pathology , biology , mathematics , botany , genus , geometry
Objectives/Hypothesis Compare cytokine levels in sinus tissue to sinus secretions from controls and chronic rhinosinusitis patients. Study Design In vitro. Methods Polyurethane foam sponges were placed into middle meati of patients with chronic rhinosinusitis without nasal polyps (CRSsNP), with polyps (CRSwNP), and controls. Sinus biopsies were then taken from the same location. Protein levels of tumor necrosis factor‐alpha (TNF‐α), interferon‐γ (IFN‐γ), and interleukins (IL) 2, 4, 6, 8, 10, and 17A were measured via cytometric bead assay for each sample. Protein values from sinus tissue and secretions were compared with Pearson's correlation between samples as well as one‐way ANOVA with posthoc t test between groups. Results Samples from 43 patients in total were examined. Mucus was measured from 10 controls, 11 CRSsNP and 10 CRSwNP, and sinus tissue was measured from 10 controls, 15 CRSsNP and 10 CRSwNP. IL‐8 and IFN‐γ levels were outside of the detectable range of the assay. Levels of secreted IL‐2, 4, 6, 10, and 17A correlated with tissue levels ( P < 0.05 for all, r > 0.49) while TNF‐α did not ( P = 0.71). CRSsNP had elevated mucus levels of IL‐2, 4, 6, 10, and 17A compared to controls. CRSwNP had elevated mucus levels of IL‐4, 6, 10, 17A, and TNF‐α compared to controls. Conclusions Cytokine levels in sinus secretions correlate with levels in sinus tissue and are elevated in CRS versus control based on Th1/Th2 skewing. Level of Evidence N/A (in vitro). Laryngoscope , 123:E72–E78, 2013