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Selectin blockade decreases postischemic recruitment of bone marrow stromal cells
Author(s) -
Lawton Brian,
Boyette Jennings R.,
Hu Melissa,
Lian Timothy S.
Publication year - 2013
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.24217
Subject(s) - stromal cell , bone marrow , fucoidan , ischemia , medicine , inferior epigastric artery , pathology , artery , chemistry , polysaccharide , biochemistry
Objectives/Hypothesis Investigate the localization mechanisms of bone marrow stromal cells following transient ischemia‐reperfusion injury in a murine flap model. Study Design Controlled laboratory study. Methods A cutaneous flap based on the inferior epigastric artery was elevated, and transient ischemia of 3.5 hours using a microvascular clamp was achieved. Fucoidan was injected intravenously 24 hours before the ischemic period. Following the period of ischemia, radiolabeled bone marrow stromal cells were injected intravenously, and radioactivity was determined postoperatively. Results Attenuation of the uptake of bone marrow stromal cells into postischemic tissue was observed in those mice treated with fucoidan as indicated by gamma counts measured in the flaps when compared with controls ( P < . 001). Conclusions Decreased uptake of radiolabeled bone marrow stromal cells into postischemic tissues pretreated with fucoidan indicates selectin‐mediated bone‐marrow stromal cell recruitment in a murine cutaneous flap model. Level of Evidence N/A. Laryngoscope , 123:2993–2995, 2013