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Effective embryoid body formation from induced pluripotent stem cells for regeneration of respiratory epithelium
Author(s) -
Otsuki Koshi,
Imaizumi Mitsuyoshi,
Nomoto Yukio,
Nomoto Mika,
Wada Ikuo,
Miyake Masao,
Omori Koichi
Publication year - 2014
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.24201
Subject(s) - embryoid body , respiratory epithelium , regeneration (biology) , induced pluripotent stem cell , epithelium , microbiology and biotechnology , stem cell , biology , chemistry , pathology , respiratory system , embryonic stem cell , anatomy , medicine , biochemistry , gene
Objectives/Hypothesis We have previously demonstrated the potential use of induced pluripotent stem (iPS) cells for regeneration of respiratory epithelium by culturing embryoid bodies (EBs). The aim of the present study was to determine the most effective conditions for EB formation from iPS cells for regeneration of respiratory epithelium. Study Design Experimental study. Methods iPS cells cultured on a gelatin‐coated dish were seeded on low‐attachment plates for generating EBs. Under several conditions including the air–liquid interface (ALI) method, with varying cell numbers and suspension times, EBs were transferred to a gelatin‐coated dish supplemented with growth factors. The shape, size, aggregation, and adhesion of EBs for iPS cell differentiation were evaluated, and the cultured tissue was histologically examined. Results EBs appropriate for differentiation were observed using 1,000 cells after 5 days of suspension culture. Respiratory epithelium‐like tissue was histologically observed. The ciliary epithelium was confirmed immunohistologically. Conclusions Based on the varying suspension times and cell numbers with the ALI method, this study presented effective conditions for EB formation from iPS cells for regeneration of respiratory epithelium. Level of Evidence NA. Laryngoscope , 124:E8–E14, 2014