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Bioengineered trachea using autologous chondrocytes for regeneration of tracheal cartilage in a rabbit model
Author(s) -
Nomoto Mika,
Nomoto Yukio,
Tada Yasuhiro,
Tani Akiko,
Otsuki Koshi,
Suzuki Ryo,
Nakamura Tatsuo,
Omori Koichi
Publication year - 2013
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.23784
Subject(s) - cartilage , medicine , regeneration (biology) , anatomy , tissue engineering , prosthesis , scaffold , surgery , biomedical engineering , microbiology and biotechnology , biology
Objectives/Hypothesis In this study, a bioengineered trachea composed of autologous chondrocytes was developed, and its effect on cartilaginous regeneration was evaluated by implantation into tracheal defects in rabbits. Study Design Prospective controlled trial in an animal model. Methods The tracheal prosthesis used in this study was composed of polypropylene (the frame) and collagen sponge (the scaffold). Chondrocytes were harvested from the costal cartilage of rabbits and seeded into the tracheal prosthesis. The bioengineered trachea, consisting of the tracheal prosthesis with chondrocytes, was implanted into surgically created tracheal defects of rabbits in the bioengineered group, and a tracheal prosthesis without chondrocytes was implanted in the control group. Results After implantation, the presence of regenerated cartilage was observed in the bioengineered trachea but not in the tracheal prosthesis without chondrocytes. Conclusions It was confirmed in this study that the implanted chondrocytes proliferated in an appropriate portion of the tracheal defect and that the partially resected tracheal cartilage was repaired with regenerated cartilaginous tissue into a ring‐shaped form as a whole. These results demonstrate the feasibility of cartilaginous regeneration using a bioengineered trachea with autologous chondrocytes Laryngoscope , 123:2195–2201, 2013 Level of Evidence NA