Premium
Characterization of human vocal fold fibroblasts derived from chronic scar
Author(s) -
Jetté Marie E.,
Hayer Supriya D.,
Thibeault Susan L.
Publication year - 2013
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.23681
Subject(s) - fibroblast , extracellular matrix , vocal folds , blot , gene expression , microbiology and biotechnology , biology , immunocytochemistry , cell growth , western blot , cell , pathology , in vitro , andrology , anatomy , gene , medicine , endocrinology , larynx , genetics
Objectives/Hypothesis: In vitro modeling of cell‐matrix interactions that occur during human vocal fold scarring is uncommon, as primary human vocal fold scar fibroblast cell lines are difficult to acquire. The purpose of this study was to characterize morphologic features, growth kinetics, contractile properties, α‐smooth muscle actin (α‐SMA) protein expression and gene expression profile of human vocal fold fibroblasts derived from scar (sVFF) relative to normal vocal fold fibroblasts (nVFF). Study Design: In vitro. Methods: We successfully cultured human vocal fold fibroblasts from tissue explants of scarred vocal folds from a 56‐year‐old female and compared these to normal fibroblasts from a 59‐year‐old female. Growth and proliferation were assessed by daily cell counts, and morphology was compared at 60% confluence for 5 days. Gel contraction assays were evaluated after seeding cells within a collagen matrix. α‐SMA was measured using western blotting and immunocytochemistry (ICC). Quantitative reverse‐transcriptase polymerase chain reaction (qRT‐PCR) was used to assess differential extracellular matrix gene expression between the two cell types. Results: sVFF were morphologically indistinguishable from nVFF. sVFF maintained significantly lower proliferation rates relative to nVFF on days 3 to 6 (day 3: P = .0138; days 4, 5, and 6: P < .0001). There were no significant differences in contractile properties between the two cell types at any time point (0 hours: P = .70, 24 hours: P = .79, 48 hours: P = .58). ICC and western blot analyses revealed increased expression of α‐SMA in sVFF as compared with nVFF at passages 4 and 5, but not at passage 6 (passage 4: P = .006, passage 5: P = .0015, passage 6: P = .8860). Analysis of 84 extracellular matrix genes using qRT‐PCR revealed differential expression of 15 genes ( P < .01). Conclusions: nVFF and sVFF displayed differences in proliferation rates, α‐SMA expression, and gene expression, whereas no differences were observed in contractile properties or morphology. Further investigation with a larger sample size is necessary to confirm these findings. Laryngoscope, 2013