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Protective effects of resveratrol on salivary gland damage induced by total body irradiation in rats
Author(s) -
Şimşek Gökçe,
Gürocak Şimay,
Karadaǧ Neşe,
Karabulut Aysun Bay,
Demirtaş Erol,
Karataş Erkan,
Pepele Eda
Publication year - 2012
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.23609
Subject(s) - resveratrol , malondialdehyde , glutathione , salivary gland , endocrinology , medicine , nitric oxide , antioxidant , histology , parotid gland , saliva , necrosis , oxidative stress , chemistry , pharmacology , pathology , biochemistry , enzyme
Objectives/Hypothesis: One of the most common acute side effects of irradiation is xerostomia, which results from damage to the salivary gland cells by direct ionization. Resveratrol is a natural compound with profound anti‐inflammatory and antioxidant properties. The purpose of the present study was to investigate the potential protective effects of resveratrol on injury to the salivary glands of rats that were exposed to total body irradiation. Study Design: An experimental study at the Inonu University School of Medicine. Methods: Twenty‐nine female rats were randomized into four groups: group 1, high‐dose (100 mg/kg) resveratrol group (n = 7); group 2, low‐dose (10 mg/kg) resveratrol group (n = 7); group 3, control (vehicle) rats (n = 7); and group 4, sham‐irradiation group (n = 8). The medications were administered as single doses, and the rats were exposed to total body irradiation 24 hours after the treatment. The animals were sacrificed the following day, and the parotid and submandibular glands were excised. Salivary gland histology and the tissue levels of glutathione (GSH), nitric oxide (NO), and malondialdehyde (MDA) were investigated. Results: The rats in group 1 showed significantly decreased acinar loss and less ductal damage and cell necrosis than those of the control group ( P < .05). Antioxidant GSH levels were significantly increased by high doses of resveratrol treatment. The tissue activities of MDA in both the parotid and submandibular glands were significantly reduced in group 1. Low‐dose resveratrol treatment did not significantly alter the tissue levels of MDA. Conclusions: Resveratrol at relatively high doses can reduce the irradiation‐dependent salivary gland damage, suggesting that this natural antioxidant may be effectively used to lessen the side effects related to salivary gland dysfunction that is induced by irradiation. Laryngoscope, 2012