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Linking expression of FOXM1 , CEP55 and HELLS to tumorigenesis in oropharyngeal squamous cell carcinoma
Author(s) -
Janus Jeffrey R.,
Laborde Rebecca R.,
Greenberg Alexandra J.,
Wang Vivian W.,
Wei Wei,
Trier Anna,
Olsen Steven M.,
Moore Eric J.,
Olsen Kerry D.,
Smith David I.
Publication year - 2011
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.22379
Subject(s) - foxm1 , carcinogenesis , stage (stratigraphy) , cancer research , transcriptome , oncology , biology , immunohistochemistry , gene expression , gene , medicine , pathology , cancer , cell cycle , genetics , paleontology
Objectives/Hypothesis: To investigate the relationship between the expression of FOXM1, CEP55 , and HELLS in oropharyngeal squamous cell carcinoma to human papillomavirus (HPV), smoking, and tumor stage. Study Design: Retrospective cohort study. Methods: Transcriptome data were analyzed from matched tumor‐normal samples taken from patients with oropharyngeal squamous cell carcinoma. Data were previously generated using deep‐sequencing techniques (mRNA‐Seq). Transcript levels of all three genes were validated using the NanoString nCounter system in a larger group of patients. Analyses were conducted to assess possible associations between expression levels and HPV infection status, smoking status, or tumor staging. Results: FOXM1, CEP55 , and HELLS were all overexpressed in oropharyngeal squamous cell carcinoma tissue when compared to normal tissue. Significant trends were found between expression levels of FOXM1, CEP55 , and HELLS and tumor staging. Tumors staged 3 or greater showed significantly higher levels of expression compared with those staged 1. No significant association or trend was found between expression of any genes of interest and etiologic subgroupings (i.e., HPV, smoking). Conclusions: FOXM1, CEP55 , and HELLS were all overexpressed in oropharyngeal squamous cell carcinoma. Gene expression is related to tumor stage. The significant association between the expression of these genes and advanced tumor stage suggest that they may play a role in tumorigenesis. Laryngoscope, 121:2598–2603, 2011