z-logo
Premium
Acute stress to excised vocal fold epithelium from reactive oxygen species
Author(s) -
Alper Rebecca,
Fu Xue,
EricksonLevendoski Elizabeth,
Zheng Wei,
Sivasankar Mahalakshmi
Publication year - 2011
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.22157
Subject(s) - reactive oxygen species , epithelium , oxygen , vocal folds , hydrogen peroxide , biology , oxidative stress , microbiology and biotechnology , pathology , chemistry , anatomy , medicine , endocrinology , biochemistry , larynx , organic chemistry
Objectives/Hypothesis: Vocal fold epithelium is exposed to reactive oxygen species from the inhaled environment and from tissue inflammation. The objective of this study was to explore the functional and structural consequences of reactive oxygen species exposure on vocal fold epithelium. Study Design: In vitro, prospective study design. Methods: Hydrogen peroxide (H 2 O 2 ), a common reactive oxygen species, was utilized in this study. Freshly excised, viable porcine vocal fold epithelia (N = 32) were exposed to H 2 O 2 or sham challenge for 2 hours. Electrophysiology, western blotting, and light microscopy were used to quantify the functional and structural effects of reactive oxygen species on vocal fold epithelia. Results: Exposure to reactive oxygen species did not significantly alter transepithelial resistance. There was a small, nonsignificant trend for decreased concentration of epithelial junctional complex protein with reactive oxygen species challenge. Minimal changes to the gross structural appearance of vocal fold epithelia were also noted. Conclusions: The stratified squamous epithelia of the vocal folds effectively defend against an acute reactive oxygen species challenge. The current study lays the groundwork for future investigations on the effects of reactive oxygen species on vocal fold epithelia that are compromised from phonotrauma.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here