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Crosstalk between adipose‐derived stem/stromal cells and vocal fold fibroblasts in vitro
Author(s) -
Kumai Yoshihiko,
Kobler James B.,
Park Hyoungshin,
LopezGuerra Gerardo,
Karajanagi Sandeep,
Herrera Victoria L.M.,
Zeitels Steven M.
Publication year - 2009
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.20149
Subject(s) - stromal cell , adipose tissue , fibroblast , vocal folds , stem cell , paracrine signalling , microbiology and biotechnology , hyaluronic acid , pathology , medicine , in vitro , biology , anatomy , receptor , biochemistry , larynx
Abstract Objectives/Hypothesis: To explore adipose‐derived stem cell/fibroblast interactions as a potential remodeling pathway for vocal fold scar. Study Design: Fibroblasts and adipose‐derived stem/stromal cells (ASCs) were cultured alone and in combination in a cell‐contact–independent paracrine system. Analyses of cell proliferation, and the production of hyaluronic acid (HA) and collagen were performed on samples collected on days 1, 3, and 7. Methods: Normal fibroblasts (NFs) were isolated bilaterally from the subepithelial lamina propria of two normal ferret vocal folds. Scar fibroblasts (SFs) were isolated from vocal folds that were electrocauterized 2 weeks before harvest. ASCs were isolated from lipoaspirated subcutaneous abdominal fat of two ferrets. A transwell cell‐contact–independent cell communication culturing system was used for coculture experiments. Cells were seeded at 50,000/well in both monoculture and coculture experiments. Results: In monoculture, SFs proliferated faster and produced less HA and more collagen than NFs at day 7 ( P < .05). In SF/ASC coculture, SF proliferation was diminished and collagen production at day 7 decreased ( P < .05). HA production did not differ between monoculture and coculture conditions. Conclusions: Normal and scar‐tissue–derived vocal fold fibroblasts maintain phenotypic differences in culture, thus validating this in vitro scar model. In co‐culture, contact‐independent crosstalk occurs between SFs and ASCa, leading to less collagen secretion. The data support the hypothesis that ASCs can induce favorable remodeling of scarred vocal folds in vivo by their interactions with endogenous fibroblasts. Laryngoscope, 2009