Premium
Transforming growth factor‐β1 shows an incremental osteoinductive dose‐response relationship
Author(s) -
Moxham J. Paul,
Wong Kevin K.,
Kibblewhite Douglas J.
Publication year - 2009
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1002/lary.20009
Subject(s) - demineralized bone matrix , transforming growth factor , animal model , dose–response relationship , dosing , matrix (chemical analysis) , extracellular matrix , pharmacology , medicine , chemistry , dbm , materials science , chromatography , biochemistry , amplifier , optoelectronics , cmos
Objective: To determine if there is a dose–response curve for TGF‐β1 in a rabbit calvarial defect model. Study Design: Controlled animal study using arms of increasing concentrations of TGF‐β1 to evaluate the osteoinductive potential of each arm. Methods: Sixteen skeletally mature New Zealand white rabbits were randomized into control and experimental arms. Incremental doses of TGF‐β1 delivered in an inactivated guanidine‐extracted demineralized bone matrix (Gu‐DBM) carrier were implanted into a critically sized calvarial defect. The animals were sacrificed at 4 weeks and histomorphometric analysis was then accomplished. Results: TGF‐β1 showed a dose–response relationship, with the higher doses chosen for this study causing more robust osteoinduction. Conclusions: In this pilot dosing study, TGF‐β1 demonstrates increasing osteoinduction with increasing dose levels in this animal model. Laryngoscope, 119:126–130, 2009