Open AccessLINC01315 promotes the aggressive phenotypes of papillary thyroid cancer cells by sponging miR‐497‐5p
Author(s) -
Ren Jian,
Zhang FengJiao,
Wang JingHong,
Tang JianDong
Publication year - 2021
Publication title -
the kaohsiung journal of medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.439
H-Index - 36
eISSN - 2410-8650
pISSN - 1607-551X
DOI - 10.1002/kjm2.12369
Subject(s) - gene knockdown , downregulation and upregulation , cancer research , medicine , thyroid cancer , microrna , thyroid carcinoma , phenotype , papillary thyroid cancer , cancer cell , cell culture , cancer , gene , thyroid , biology , genetics
Dysregulation of the long intergenic noncoding RNA 01315 (LINC01315) has recently been demonstrated in cancer. However, the role of LINC01315 in papillary thyroid cancer (PTC) has not been determined. We attempted to determine the function of LINC01315 in PTC. The levels of LINC01315 were higher in thyroid carcinoma tissues and cell lines compared with that in noncancerous tissues or normal cells, respectively. LINC01315 knockdown significantly inhibited the in vitro colony formation and invasion of PTC cells. Upregulation of LINC01315 produced opposite effects. Bioinformatic analysis and luciferase reporter assays indicated direct binding of miR‐497‐5p to LINC01315. Gain‐ and loss‐of‐function assays indicated that miR‐497‐5p acts as a suppressive miRNA in PTC. Furthermore, LINC01315 facilitated the growth and invasion of PTC cells by sponging miR‐497‐5p. Our results demonstrated the critical role of the LINC01315‐miR‐497‐5p axis in the growth and invasion of PTC cells.