Open AccessCASC2 inhibits the growth, migration, and invasion of thyroid cancer cells through sponging miR ‐18a‐5p/ FIH1 axis
Author(s) -
Liu QiYu,
Gao LingYi,
Xu Lin,
Zhang XiaoLong,
Zhang LiJun,
Gong XiaoLei,
Luo ShiBi,
Zhao Rong,
Cheng RuoChuan
Publication year - 2021
Publication title -
the kaohsiung journal of medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.439
H-Index - 36
eISSN - 2410-8650
pISSN - 1607-551X
DOI - 10.1002/kjm2.12331
Subject(s) - competing endogenous rna , downregulation and upregulation , thyroid cancer , microrna , medicine , cancer research , long non coding rna , cancer , thyroid , gene , biology , genetics
Long noncoding RNA (lncRNA) Cancer Susceptibility 2 (CASC2) has been proved to contribute to the development of cancers. However, the mechanism behind the action of CASC2 in thyroid cancer is not quite clear. We demonstrated that CASC2 was downregulated in thyroid cancer. We noted that CASC2 overexpression restrained the growth, migration, and invasion of thyroid cancer cells, whereas CASC2 depletion caused opposite trends. Bioinformatics analysis predicted that hypoxia inducible factor 1 subunit alpha inhibitor (FIH‐1) was potentially targeted by miR‐18a‐5p, which was confirmed by luciferase reporter assay. Upregulation of FIH‐1 abrogated the promotive effect of miR‐18a‐5p on the growth and invasion of thyroid cancer cells. In addition, CASC2 serves as a competing endogenous RNA (ceRNA) and a ''sponge'' for miR‐18a‐5p, thereby regulating the expression of FIH‐1. These data elucidated the CASC2/miR‐18a‐5p ceRNA network in thyroid cancer pathogenesis.