Open AccessIn vitro effect of caveolin‐1 as a slow‐release material on bone‐tendon junction healing: A comparative study
Author(s) -
Liang LinLin,
Su ZhengBing
Publication year - 2019
Publication title -
the kaohsiung journal of medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.439
H-Index - 36
eISSN - 2410-8650
pISSN - 1607-551X
DOI - 10.1002/kjm2.12022
Subject(s) - fibrocartilage , medicine , tendon , ultimate tensile strength , bone healing , anatomy , biomedical engineering , surgery , pathology , materials science , osteoarthritis , alternative medicine , metallurgy , articular cartilage
Bone tendon junction injury is hard to cure because of its special anatomical structure, and the treatment applied for bone‐tendon junction injury cannot result in the perfect vascular regeneration and restoration of the fibrocartilage zone. In this article, we aim to explore the effect of caveolin‐1 as a slow‐release material on bone‐tendon junction healing. Seventy‐two New Zealand rabbits were randomly selected and assigned into the experimental, sham‐operated and control groups (n = 24). Caveolin‐1 microspheres and microcapsule were developed as drug delivery system. At the 4th, 8th, and 12th weeks after surgery, quadriceps muscle patella‐patellar tendon (QMPPT) was obtained from each rabbit to observe the tendon‐to‐bone tunnel healing, and X‐ray examination, histological examination and biomechanical testing were applied for evaluating new bone formation. As the X‐ray showed, caveolin‐1 increased the new bone area at each time point. At the 4th and 8th weeks after surgery, the rabbit treated with caveolin‐1 slow release material showed repair of fibrocartilage. According to the biomechanical results, the cross‐sectional area, breaking load and ultimate tensile strength were increased along with time. At the same time point, caveolin‐1 increased the ultimate tensile strength. Our study demonstrates that caveolin‐1 as a slow‐release material could accelerate bone‐tendon junction healing by promoting the formation of the transition zone.