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Influence of Amine‐Based Cationic Gemini Surfactants on Catalytic Activity of α‐Chymotrypsin
Author(s) -
Verma Santosh Kumar,
Ghritlahre Bhupendra Kumar,
Ghosh Kallol K,
Verma Rameshwari,
Verma Shekhar,
Zhao Xiujian
Publication year - 2016
Publication title -
international journal of chemical kinetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.341
H-Index - 68
eISSN - 1097-4601
pISSN - 0538-8066
DOI - 10.1002/kin.21032
Subject(s) - chemistry , cationic polymerization , amine gas treating , chymotrypsin , catalysis , organic chemistry , combinatorial chemistry , enzyme , trypsin
The α‐chymotrypsin activity was tested in aqueous media with the presence of novel cationic amine–based gemini surfactant, with different spacer chain lengths and head group size, and also compared with the cationic cetyltrimethylammonium bromide (CTAB) and cetyltriphenylphosphonium bromide (CTPB) surfactants and aqueous buffer only. The p ‐nitrophenyl acetate (PNPA) hydrolysis rate was monitored in the presence of the surfactant concentration at 30°C. Most of these gemini surfactants gave higher catalytic activity as compared to cationic CTAB and CTPB. The highest superactivity was measured in the presence of gemini 16‐12‐16, [dodecanediyl‐1,12‐bis(cetyldimethylammonium bromide)] surfactant at pH 7.5. The catalytic reaction follows the Michaelis–Menten mechanism. The catalytic rate constants, k cat , show the same profile that the catalytic affinity; K M being enhanced with increasing space chain length. The results are favorable for considering that the amine‐based gemini surfactant influences more than both the aqueous and cationic micellar media.