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Kinetic Study of Hydrogen–Deuterium Exchange of Glutamic Acid in Deuterated Hydrochloric Acid Solution
Author(s) -
She Anqi,
Gang Hongze,
Yang Shizhong,
Mu Bozhong
Publication year - 2013
Publication title -
international journal of chemical kinetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.341
H-Index - 68
eISSN - 1097-4601
pISSN - 0538-8066
DOI - 10.1002/kin.20760
Subject(s) - chemistry , deuterium , hydrogen–deuterium exchange , hydrochloric acid , arrhenius equation , methylene , activation energy , hydrogen , kinetics , chemical kinetics , nuclear chemistry , analytical chemistry (journal) , inorganic chemistry , medicinal chemistry , organic chemistry , nuclear physics , physics , quantum mechanics
The kinetics of the hydrogen–deuterium (H–D) exchange at both the methine (alpha) and methylene (gamma) positions of glutamic acid in deuterated hydrochloric acid solution has been studied in the temperature range of 383–433 K by 1 H NMR detection. The reaction rates of H–D exchange at the two positions were described by applying multivariable linear regression (MLR) analysis and are determined as v = k [Glu] 3.3 [D 3 O + ] 1.5 mol L −1 h −1 with k = 3.52 × 10 16 × exp (–1.37 × 10 5 / RT ) mol −3.8 L h −1 for the alpha position as well as v = k [Glu] 1.0 [D 3 O + ] 0.45 mol L −1 h −1 with k = 1.77 × 10 12 × exp (–0.99 × 10 5 / RT ) mol −0.45 L h −1 for the gamma position. The Arrhenius activation energy ( E a ) at the gamma position is less than that at the alpha position, which implies that the deuteration reaction at the gamma position proceeded more easily.