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Effects of head group of cationic surfactants on the hydrolysis of p ‐nitrophenyl acetate catalyzed by α‐chymotrypsin
Author(s) -
Ghosh Kallol K.,
Verma Santosh Kumar
Publication year - 2009
Publication title -
international journal of chemical kinetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.341
H-Index - 68
eISSN - 1097-4601
pISSN - 0538-8066
DOI - 10.1002/kin.20408
Subject(s) - bromide , chemistry , cationic polymerization , pulmonary surfactant , hydrolysis , catalysis , counterion , chymotrypsin , reaction rate constant , residue (chemistry) , medicinal chemistry , kinetics , nuclear chemistry , inorganic chemistry , organic chemistry , enzyme , trypsin , ion , biochemistry , physics , quantum mechanics
The kinetics of hydrolysis of p ‐nitrophenyl acetate catalyzed by α‐chymotrypsin (α‐CT) has been studied in the presence of several cationic surfactants having different head groups maintaining the dodecyl hydrophobic residue and bromide counterion. The enzyme activity was tested in the presence of dodecyl trimethylammonium bromide (DTAB), dodecylpyridinium bromide (DPB), dodecyldimethylethanolammonium bromide (DDMEAB), dodecyldiethylethanolammonium bromide (DDEEAB), benzyldimethyldodecylammonium bromide (BDDAB), and dodecyltriphenylphosphonium bromide (DTPB) surfactants. The extent of superactivity depends upon head groups of surfactants. The activity of α‐CT depends on the surfactant concentration and it varies with the surfactant head group dimensions (DTPB > DDEEAB > DTAB > BDDAB > DDMEAB > DPB). For all surfactants, DTPB exhibits highest superactivity. The effects of surfactants on the apparent kinetic parameters like Michaelis constant K m and the catalytic constant k cat have been determined. © 2009 Wiley Periodicals, Inc. Int J Chem Kinet 41: 377–381, 2009