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Effects of Low‐Intensity Pulsed Ultrasound on Myelosuppression of Rats Induced by Chemotherapy Drugs With Cell Cycle Specificity
Author(s) -
Wang Wei,
Chen Junlin,
Luo Dong,
Chen Jinyun,
Xu Haopeng,
Chen Wenzhi,
Wang Yan
Publication year - 2021
Publication title -
journal of ultrasound in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 91
eISSN - 1550-9613
pISSN - 0278-4297
DOI - 10.1002/jum.15562
Subject(s) - medicine , low intensity pulsed ultrasound , etoposide , granulocyte colony stimulating factor , chemotherapy , docetaxel , pathology , urology , ultrasound , andrology , therapeutic ultrasound , radiology
Objectives To explore the ameliorating effects of low‐intensity pulsed ultrasound (LIPUS) on Sprague Dawley rat myelosuppression induced by cell cycle specificity drugs (docetaxel, mitotic phase sensitive; and etoposide, gap 2 phase sensitive). Methods Rats were respectively administered docetaxel (100 mg/kg) or etoposide (110 mg/kg) by intraperitoneal injection for 4 consecutive days. Then the rats were divided randomly into a LIPUS group and a non‐LIPUS group. In the LIPUS group, the right femoral metaphysis of rats was treated by LIPUS (acoustic intensity, 200 mW/cm 2 ; frequency, 0.3 MHz; and duty cycle, 20%) for 20 minutes on 7 consecutive days from day 5. The rats of the non‐LIPUS group were treated without ultrasound output. A blood cell count, an enzyme‐linked immunosorbent assay, a real‐time quantitative polymerase chain reaction, and hematoxylin–eosin staining were applied to detect the results. Results Low‐intensity pulsed ultrasound significantly promoted the counts of bone marrow nucleated cells, white blood cells, immunoglobulin A (IgA), IgG, granulocyte colony‐stimulating factor, stem cell factor, and intercellular cell adhesion molecule 1 and reduced the counts of vascular cell adhesion molecule 1 whether in the docetaxel or etoposide group ( P < .05). Low‐intensity pulsed ultrasound only increased the expression level of IgM in the docetaxel group but decreased the level of interleukin 6 in the etoposide group ( P < .05). Conclusions Low‐intensity pulsed ultrasound has potential to be a noninvasive treatment for myelosuppression caused by different cell cycle–sensitive chemotherapy drugs.