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Hepatic Microwave Ablation–Induced Tumor Destruction and Animal End Point Survival Can Be Improved by Suppression of Heat Shock Protein 90
Author(s) -
Zhai Hongyan,
Zhou Qunfang,
Dou Jianping,
Liu Fangyi,
Zhu Xinyuan,
Yu Jie,
Liang Ping
Publication year - 2020
Publication title -
journal of ultrasound in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 91
eISSN - 1550-9613
pISSN - 0278-4297
DOI - 10.1002/jum.15212
Subject(s) - medicine , microwave ablation , coagulative necrosis , heat shock protein , apoptosis , hsp90 inhibitor , in vivo , tumor necrosis factor alpha , thermal ablation , pathology , cancer research , ablation , hsp90 , biology , biochemistry , microbiology and biotechnology , gene
Objectives To investigate the effect of heat shock protein 90 (HSP90) modulation on tumor necrosis, apoptosis, tumor growth delay, and end point survival by combining microwave ablation (MWA) with an HSP90 inhibitor in a nude mouse model. Methods This study was approved by the Ethics Committee. Forty mice with HepG2 subcutaneous xenograft tumors (10 ± 1 mm) were randomized into 4 groups: (1) no treatment, (2) MWA only, (3) the HSP90 inhibitor ganetespib only, and (4) ganetespib combined with MWA. Tumors were harvested 24 hours after treatment, and gross coagulation diameters were measured. The effect of ganetespib on HSP90 and caspase 3 expression in the periablational rim was assessed. Another 40 mice with the same tumors and groupings were observed after treatment. Tumor growth curve and Kaplan‐Meier survival analyses were performed with a tumor diameter of 2.2 cm and 40 days of survival as the defined survival end points. Results Combination treatment significantly increased the coagulation size compared to tumors treated with MWA or ganetespib alone ( P < 0.05). The combination of MWA and ganetespib decreased HSP90 expression and increased cleaved caspase 3 expression 24 hours after treatment. Compared with MWA or ganetespib only, combination treatment could lengthen the end point survival and reduce the tumor growth rate. Conclusions Modulation of HSP production can improve MWA‐induced tumor apoptosis and destruction, reduce residual tumor growth rates, and prolong end point survival.