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Integrin Antibody Decreases Deformability of Patient‐Derived Pre‐B Acute Lymphocytic Leukemia Cells as Measured by High‐Frequency Acoustic Tweezers
Author(s) -
Liu HsiaoChuan,
Gang Eun Ji,
Kim Hye Na,
AbdelAzim Nour,
Chen Ruimin,
AbdelAzim Hisham,
Shung K. Kirk,
Kim YongMi
Publication year - 2020
Publication title -
journal of ultrasound in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 91
eISSN - 1550-9613
pISSN - 0278-4297
DOI - 10.1002/jum.15139
Subject(s) - integrin , medicine , stromal cell , antibody , cell adhesion molecule , leukemia , chronic lymphocytic leukemia , adhesion , cell adhesion , immunology , cancer research , microbiology and biotechnology , receptor , materials science , biology , composite material
Objectives This article reports a study of cell mechanics in patient‐derived (primary) B‐cell acute lymphocytic leukemia (ALL) cells treated with antibodies against integrins. Leukemia cell adhesion to stromal cells mediates chemotherapeutic drug resistance, also known as cell adhesion‐mediated chemotherapeutic drug resistance. We have previously shown that antibodies against integrin α 4 and α 6 adhesion molecules can de‐adhere ALL cells from stromal cells or counter‐receptors. Because drug‐resistant cells are more deformable, as evaluated by single‐beam acoustic tweezers, we hypothesized that changes in cell mechanics might contribute to the de‐adhesive effect of integrin‐targeting antibodies. Methods In this study, the deformability of primary pre‐B ALL cells was evaluated by single‐beam acoustic tweezers after treatments with the de‐adhering antibody Tysabri or P5G10 against integrin α 4 and α 6 adhesion molecules. Results We demonstrated that primary ALL cells treated with P5G10 expressed decreased deformability compared with immunoglobulin G 1 ‐treated control cells ( P < .05). Tysabri did not show an effect on deformability ( P > .05). Conclusions These results suggest that decreased deformability is associated with an integrin α 6 blockade. Further assessments of the functional roles of deformability and integrin blockades in B‐ALL cell drug resistance and deformability, respectively, are necessary.