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Quantitative Analysis of Hepatic Toxicity in Rats Induced by Inhalable Silica Nanoparticles Using Acoustic Radiation Force Imaging
Author(s) -
Cao Wen,
Zhou Yajing,
Niu Yingmei,
Zhu Xiaoli,
Song Yuguo,
Guo Ruijun
Publication year - 2017
Publication title -
journal of ultrasound in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 91
eISSN - 1550-9613
pISSN - 0278-4297
DOI - 10.1002/jum.14219
Subject(s) - medicine , nanoparticle , saline , alanine aminotransferase , toxicity , nuclear medicine , nanotechnology , materials science
Objectives The purposes of this study were to verify whether inhalable silicon dioxide (SiO 2 ) nanoparticles could induce hepatic injury and to investigate the relationship between the exposure time and SiO 2 nanoparticle dosage by using acoustic radiation force impulse imaging (ARFI). Methods A total of 72 rats were randomly separated into 9 groups with 8 in each: blank control group, 0.9% normal saline group, polyacrylate (PPE) group, 25%, 50%, and 100% SiO 2 groups, and 25%, 50%, and 100% SiO 2 /PPE groups with inhaled SiO 2 nanoparticle concentrations similar to the SiO 2 groups. After successful modeling and design, the hepatic shear wave velocity (SWV) values of the 9 groups were obtained on days 3, 7, 14, 21, and 28 by using ARFI, and the intragroup and intergroups differences in the SWVs were compared. The serum alanine aminotransferase (ALT) and aspartate aminotransferase were tested and compared on day 28. Hepatic tissues were collected for histologic observation on day 28. Results The pathologic results verified that inhalable SiO 2 nanoparticles could induce hepatic injury. Compared with the control group, the hepatic SWV and serum ALT values in the SiO 2 groups and SiO 2 /PPE groups were elevated ( P < .05). The dosage and exposure time of SiO 2 played a key role in the elevation of the SWV in the SiO 2 and SiO 2 /PPE groups. The correlation between the ALT level and SWV was significant on day 28 ( P < .05). Conclusions Inhalable SiO 2 nanoparticles and SiO 2 /PPE were able to induce hepatic injury in rats. Using ARFI to evaluate hepatic toxicity induced by SiO 2 nanoparticles was effective in this study.