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Front Cover: Absolute protein assay for the simultaneous quantification of two epoxide hydrolases in rats by mass spectrometry–based targeted proteomics
Author(s) -
Wu Ting,
Xi Xiaoyun,
Chen Ying,
Jiang Chao,
Zhang Qian,
Dai Guoliang,
Bai Yongtao,
Zhang Weidong,
Ni Ting,
Zou Jiandong,
Ju Wenzheng,
Xu Meijuan
Publication year - 2021
Publication title -
journal of separation science
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.202170141
Subject(s) - chemistry , chromatography , proteomics , mass spectrometry , front cover , quantitative proteomics , tandem mass spectrometry , shotgun proteomics , biochemistry , cover (algebra) , mechanical engineering , engineering , gene
DOI: 10.1002/jssc.202100066 The cover picture shows a schematic diagram of a liquid chromatography with tandem mass spectrometry method for analyzing microsomal and soluble epoxide hydrolase in rat heart, kidney, and liver S9 samples. Protein quantification was realized through targeted proteomics for the determination of trypsin‐specific surrogate peptides after digestion. A multiple reaction monitoring method in a positive ionization mode was used for the simultaneous detection of the peptides. The validated proteomics method is accurate, rapid, and sensitive and can be applied for the efficient and simultaneous determination of the two enzymes compared to antibody‐based assays.