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A convenient separation strategy for fungal anthraquinones by centrifugal partition chromatography
Author(s) -
Hammerle Fabian,
Zwerger Michael,
Höck Anja,
Ganzera Markus,
Peintner Ursula,
Siewert Bianka
Publication year - 2022
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.202100869
Subject(s) - chromatography , anthraquinones , ethyl acetate , fractionation , basidiomycota , methanol , chemistry , acetic acid , pigment , column chromatography , organic chemistry , biology , botany
As recently shown, some fungal pigments exhibit significant photoactivity turning them into promising agents for the photodynamic treatment of microbial infections or malignant diseases. In the present study, a separation strategy for fungal anthraquinones was developed based on centrifugal partition chromatography. A suitable method was explored employing a methanolic extract of the fruiting bodies of Cortinarius sanguineus (Agaricales, Basidiomycota). An excellent fractionation was achieved using a biphasic solvent system comprising chloroform/ethyl acetate/methanol/water/acetic acid (3:1:3:2:1, v/v/v/v/v) operating in ascending mode. Experiments on an analytical scale with extracts of closely related Cortinarius species exhibited broad applicability of the devised system. Up to six pigments could be purified directly from the crude extract. Preparative‐scale fractionation of the methanol extracts of C. malicorius and C. sanguineus demonstrated that up‐scaling was possible without compromising selectivity.