Premium
Chiral separation using cyclodextrins as mobile phase additives in open‐tubular liquid chromatography with a pseudophase coating
Author(s) -
Yu Raymond B.,
Quirino Joselito P.
Publication year - 2022
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.202100835
Subject(s) - chemistry , chromatography , cyclodextrin , resolution (logic) , ionic liquid , organic chemistry , catalysis , artificial intelligence , computer science
The chiral separation of various analytes (dichlorprop, mecoprop, ibuprofen, and ketoprofen) was demonstrated with different cyclodextrins as mobile phase additives in open‐tubular liquid chromatography using a stationary pseudophase semipermanent coating. The stable coating was prepared by a successive multiple ionic layer approach using poly(diallyldimethylammonium chloride), polystyrene sulfonate, and didodecyldimethyl ammonium bromide. Increasing concentrations (0–0.2 mM) of various native and derivatized cyclodextrins in 25 mM sodium tetraborate (pH 9.2) were investigated. Chiral separation was achieved for the four test analytes using 0.05–0.1 mM β‐cyclodextrin (resolution between 1.11 and 1.34), γ‐cyclodextrin (resolution between 0.78 and 1.27), carboxymethyl‐β‐cyclodextrin (resolution between 1.64 and 2.59), and 2‐hydroxypropyl‐β‐cyclodextrin (resolution between 0.71 and 1.76) with the highest resolutions obtained with 0.1 mM carboxymethyl‐β‐cyclodextrin. %RSD values were <10%. This is the first demonstration of chiral open‐tubular liquid chromatography using achiral chromatographic coatings and cyclodextrins as mobile phase additives.