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Analysis of adsorption energy distribution in selected hydrophilic‐interaction chromatography systems with amide, amine, and zwitterionic stationary phases
Author(s) -
Ziobrowski Piotr,
Chutkowski Marcin,
Przywara Mateusz,
Zapała Lidia,
KosińskaPezda Małgorzata,
Zapała Wojciech
Publication year - 2021
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.202100160
Subject(s) - adsorption , chemistry , sorption , amine gas treating , acetonitrile , amide , phenol , methanol , chromatography , solvent , organic chemistry
Adsorption mechanisms of caffeine, quercetin, and phenol as test substances in various chromatographic systems have been analyzed. The investigations were conducted using three different chromatographic columns packed with polar bonded stationary phases, that is, amide, amine, and zwitterionic. Methanol–water and acetonitrile–water systems with different organic solvent contents have been used as mobile phases. On the basis of adsorption isotherms obtained for the tested systems, Scatchard plots and adsorption energy distributions have been determined. The most likely retention mechanisms have been discussed. The results of investigations indicate that (i) the surfaces of tested adsorbents are energetically heterogeneous, and (ii) the main role in sorption mechanism is played by low‐energy sites.