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Separation and purification of magnoflorine, spinosin, and 6‴‐feruloyspinosin from Ziziphi Spinosae Semen by high‐speed counter‐current chromatography
Author(s) -
Xu Hao,
Niu Yuyu,
Sun Yan,
Xia Menglin,
Lin Daozheng,
Qiao Wei
Publication year - 2021
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.202100122
Subject(s) - chemistry , chromatography , semen , countercurrent chromatography , high performance liquid chromatography , biology , genetics
In the study, high‐speed counter‐current chromatography was used for separation and purification of magnoflorine, spinosin, and 6‴‐feruloyspinosin from Ziziphi Spinosae Semen . With n ‐butanol‐ethyl acetate‐water (2:3:5, v/v) as the optimum solvent system, about 75 mg of magnoflorine, 110 mg of spinosin, and 40 mg of 6‴‐feruloyspinosin were isolated from 0.5 g of crude extract of Z. Spinosae Semen , with the purity of 95.7, 97.2, and 96.4%, respectively. The chemical structures were identified by MS and NMR spectroscopy. In addition, the antidepressant activity of the isolated components was evaluated by PC12 cells injury model and chronic unpredictable mild stress depression mouse model. The results showed that high‐speed counter‐current chromatography could be used to realize the one‐time rapid preparation and separation of magnoflorine, spinosin, and 6‴‐feruloyspinosin from Z. Spinosae Semen and compatibility of these isolated components has certain antidepressant activity.