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Development and validation of a rapid high‐performance thin‐layer chromatographic method for quantification of gallic acid, cinnamic acid, piperine, eugenol, and glycyrrhizin in Divya‐Swasari‐Vati, an ayurvedic medicine for respiratory ailments
Author(s) -
Balkrishna Acharya,
Sharma Priyanka,
Joshi Monali,
Srivastava Jyotish,
Varshney Anurag
Publication year - 2021
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.202100096
Subject(s) - glycyrrhizin , chromatography , piperine , medicine , traditional medicine , scutellaria baicalensis , chemistry , pharmacology , traditional chinese medicine , alternative medicine , pathology
Divya‐Swasari‐Vati is a calcium containing polyherbal ayurvedic medicine prescribed for the lung‐related ailments observed in the current pandemic of Severe Acute Respiratory Syndrome Coronavirus 2 infections. The formulation is a unique quintessential blend of nine herbs cited in Ayurvedic texts for chronic cough and lung infection. Analytical standardization of herbal medicines is the pressing need of the hour to ascertain the quality compliance. This persuaded us to develop a simple, rapid, and selective high‐performance thin‐layer chromatographic method for Divya‐Swasari‐Vati quality standardization. The developed method was validated for the quantification of marker components, gallic acid, cinnamic acid, piperine, eugenol and glycyrrhizin, against reference standards in five different batches of Divya‐Swasari‐Vati. The analytes were identified by visualization at 254 nm, and by matching their retention factor with authentic standards. The developed method was validated as per the guidelines recommended by the International Council for Harmonization for parameters like, linearity, limit of detection, limit of quantification, accuracy, and precision. Therefore, the developed novel high‐performance thin‐layer chromatographic process could be employed for rapid standardization of Divya‐Swasari‐Vati and other related herbal formulation, which would aid in quality manufacturing and product development.