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Liquid chromatography–tandem mass spectrometry measurement of 26 steroid hormones in human serum and plasma samples
Author(s) -
Jia Yongjuan,
Liu Xingli,
Xu Li,
Zhao Jinbao,
Ni Junjun,
Zhang Yuanyuan
Publication year - 2021
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.202100091
Subject(s) - chromatography , chemistry , analyte , steroid , ethylenediaminetetraacetic acid , tandem mass spectrometry , mass spectrometry , solid phase extraction , extraction (chemistry) , liquid chromatography–mass spectrometry , quantitative analysis (chemistry) , sample preparation , bioanalysis , hormone , biochemistry , organic chemistry , chelation
The measurement of steroid hormones provided critical information in the clinical evaluation of endocrine disorders. In this study, we developed a high‐throughput solid‐phase extraction method for the analysis of 26 steroids in human serum and plasma samples by liquid chromatography–tandem mass spectrometry. Chromatographic conditions and sample preparation were optimized to achieve good separation and maximum sensitivity for these analytes. Under the optimum conditions, good linearities were achieved in the quantitative range for each steroid hormone with the correlation coefficients ( r ) larger than 0.99. The limits of quantitation of the method were in the range from 0.0005 to 0.7901 ng/mL. The recoveries were in the range of 87.2–114.2% with intra‐ and interday precision lower than 9.94%. This method has already been applied to series of samples from clinical trials, and there was no significant difference between serum and ethylenediaminetetraacetic acid plasma samples.