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Bioactivity‐guided separation of antifungal compounds by preparative high‐performance liquid chromatography
Author(s) -
Li Zhidong,
Dai Zhuoshun,
Jiang Dasen,
Dai Yingping,
Jin Yu,
Fu Qing,
Liang Xinmiao
Publication year - 2021
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.202100072
Subject(s) - sheath blight , chromatography , high performance liquid chromatography , chemistry , antifungal , fraction (chemistry) , fermentation , rhizoctonia solani , biology , botany , organic chemistry , microbiology and biotechnology
Abstract Bioactivity‐guided chromatographic methods are of great significance for the isolation of the active compounds in complex samples. In this study, four anti‐fungal compounds were located by activity screening and successfully isolated from a microbial fermentation sample by preparative high‐performance liquid chromatography. Separation performance of columns including C18, positively charged C18, negatively charged C18 and C8 were firstly investigated. And it showed a better capacity of mixed‐mode stationary phases for retention and separation. Therefore, the positively charged C18 column was used to separate the sample into several fractions, among which the active one was identified by the antifungal test. And then the active fraction was enriched and separated again by successively using the negatively charged C18 and C8 columns to obtain four compounds, which were identified as polyoxins A, K, F and H. With activity verification, four polyoxins were found to have good inhibitory effects against the three fungal plant diseases including rice sheath blight, tomato grey mould disease, and apple spot leaf disease.