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A new HPLC‐MS/MS analytical method for quantification of tazobactam, piperacillin, and meropenem in human plasma
Author(s) -
Krnáč Dušan,
Reiffová Katarína,
Rolinski Boris
Publication year - 2021
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.202100067
Subject(s) - chromatography , chemistry , formic acid , protein precipitation , tazobactam , meropenem , piperacillin , elution , high performance liquid chromatography , antibacterial agent , imipenem , bacteria , biochemistry , antibiotic resistance , biology , pseudomonas aeruginosa , genetics , antibiotics
A simple and fast high‐performance liquid chromatography with tandem mass spectrometry method for quantification of tazobactam, piperacillin, and meropenem in human plasma has been developed and validated. Simple sample preparation with a volume of 10 μL was done by protein precipitation with a mixture of methanol–acetonitrile–water (6:2:2, v/v/v). Chromatographic separation was achieved on a Luna column with a precolumn security guard by gradient elution using a mobile phase consisting of water with the addition of 0.1% formic acid (component A) and mixture methanol–acetonitrile (8:2, v/v) with the addition of 0.1% formic acid (component B). The run time was 2.7 min. The lower limits of detection and lower limits of quantification were for piperacillin 0.03 and 0.1 mg/L, for meropenem 0.04 and 0.2 mg/L and for tazobactam 0.16 and 0.5 mg/L. The validated method was used for therapeutic monitoring of tazobactam, piperacillin, and meropenem in samples of patients treated in the intensive care unit.