Premium
Characterization of galacto‐oligosaccharides using high‐performance anion exchange chromatography‐tandem mass spectrometry
Author(s) -
Chen Yongjing,
Liu Yan
Publication year - 2021
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.202100064
Subject(s) - chemistry , mass spectrometry , tandem mass spectrometry , oligosaccharide , chromatography , collision induced dissociation , dissociation (chemistry) , tandem , protein mass spectrometry , analytical chemistry (journal) , organic chemistry , materials science , composite material
The analysis of complex oligosaccharide mixtures remains a challenge in the field of analytical chemistry. In this work, two commercial galacto‐oligosaccharides samples were characterized using high‐performance anion exchange chromatography coupled to mass spectrometry. The isomeric oligosaccharides were resolved with high resolution. The structures of the individual isomers with a degree of polymerization up to 6 were analyzed using targeted selected ion monitoring with data‐dependent tandem mass spectrometry, with additional in‐source collision‐induced dissociation.