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Pharmacokinetic studies of six major 2‐(2‐phenylethyl) chromones in rat plasma using ultra high performance liquid chromatography with tandem mass spectrometry after oral administration of agarwood ethanol extract
Author(s) -
Xie Bin,
Luo Huitai,
Huang Xiaolan,
Huang Fang,
Zhang Qiuyan,
Wu Xuefeng,
Zhou Xi,
Wu Huiqin
Publication year - 2021
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.202100053
Subject(s) - chemistry , chromatography , agarwood , formic acid , pharmacokinetics , high performance liquid chromatography , tandem mass spectrometry , mass spectrometry , analyte , chromone , selected reaction monitoring , electrospray ionization , liquid chromatography–mass spectrometry , pharmacology , stereochemistry , medicine , alternative medicine , pathology
In this study, a simple, quick, sensitive and reliable method utilizing ultra‐high performance liquid chromatography with tandem mass spectrometry method was validated for simultaneous quantification of six main 2‐(2‐phenylethyl) chromones, including agarotetrol, isoagarotetrol, (5S,6R,7R,8S)‐5,6,7,8‐tetrahydroxy‐(4‐methoxyphenethyl)‐5,6,7,8‐tetrahydro‐4H‐chromen‐4‐one, 8‐chloro‐2‐(2‐phenyl ethyl)‐5,6,7‐trihydroxy‐5,6,7,8‐tetrahydrochromone, 6,7‐dimethoxy‐2‐(2‐phenylethyl) chromone, and 2‐(2‐phenylethyl) chromone in rat plasma after oral administration of agarwood ethanol extract. Separation was performed on a Waters ACQUITY UPLC BEH C 18 column (2.1 × 100 mm, 1.7 μm) using gradient elution with mobile phase of 0.2% formic acid‐water and acetonitrile. The tandem mass was performed in the multiple reaction monitoring mode with positive ionization. The calibration curves indicated good linearity ( r 2 > 0.99) over the corresponding concentration range. The precision and accuracy were within the acceptable range. Mean absolute recoveries of all analytes were between 73.31% and 94.76%, and the relative standard deviations of matrix effects were not higher than 15%. The six analytes were proven to be stable during sample storage and analysis procedures. The validated method was successfully applied to pharmacokinetic study of six 2‐(2‐phenylethyl) chromones in rat after oral administration of agarwood ethanol extract for the first time. This study could serve as a reference and provide theoretical guidance for further pharmacodynamic research and clinical applications of agarwood.