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Optimization of an ultrasound‐assisted alcohol‐based deep eutectic solvent dispersive liquid‐phase microextraction for separation and preconcentration of quercetin in wine and food samples with response surface methodology
Author(s) -
Altunay Nail,
Elik Adil,
Unal Yener,
Kaya Savaş
Publication year - 2021
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.202100048
Subject(s) - chromatography , central composite design , detection limit , deep eutectic solvent , extraction (chemistry) , repeatability , quercetin , response surface methodology , wine , chemistry , solid phase microextraction , reproducibility , solvent , materials science , eutectic system , analytical chemistry (journal) , gas chromatography–mass spectrometry , mass spectrometry , organic chemistry , food science , alloy , antioxidant
We optimized ultrasound‐assisted alcohol‐based deep eutectic solvent dispersive liquid‐phase microextraction for separation and preconcentration of quercetin in wine and food samples by experimental design based on central composite design. Five different alcohol‐based deep eutectic solvents were prepared and tested for quercetin extraction. The effect of important parameters and matrix components were optimized. After optimization, the determination of quercetin was performed at 385 nm using s pectrophotometry. Analytical data such as detection limit, working range and preconcentration factor were found as 6.1 μg/L, 20–850 μg/L, and 120, respectively. The selectivity of the optimized extraction conditions for quercetin was investigated in the presence of different matrices. The validation of the method was investigated by reproducibility, repeatability and recovery studies, as well as by comparing the analytical results obtained from real samples with the reference method. Lastly, the recommended procedure was successfully applied for the extraction and quantification of quercetin in wine and food samples.