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Back Cover: Ultra‐high performance separation of basic compounds on reversed‐phase columns packed with fully/superficially porous silica and hybrid particles by using ultraviolet transparent hydrophobic cationic additives
Author(s) -
Manetto Simone,
Mazzoccanti Giulia,
Ciogli Alessia,
Villani Claudio,
Gasparrini Francesco
Publication year - 2020
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.202070094
Subject(s) - elution , chemistry , analyte , chromatography , phase (matter) , porosity , cationic polymerization , ultraviolet , hydrophobic effect , chemical engineering , materials science , organic chemistry , optoelectronics , engineering
DOI: 10.1002/jssc.201901333 The cover shows that, working at low pH elution conditions, the tetrabutylammonium additive produces a dynamic positive charged layer on stationary phase stabilized by hydrophobic and electrostatic interactions, and masks unfavourable interactions with silanols. In this way, the basic analyte amitriptyline, positively charged in the elution conditions, can elute faster, thanks to electrostatic repulsion, from chromatographic column providing very symmetrical peak. The approach has been specifically designed for basic analytes in RP‐UHPLC regime.