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Purification of linarin and hesperidin from Mentha haplocalyx by aqueous two‐phase flotation coupled with preparative HPLC and evaluation of the neuroprotective effect of linarin
Author(s) -
Zeng Jiajia,
Hu Weilun,
Li Hao,
Liu Jiangang,
Zhang Peng,
Gu Yanxiang,
Yu Yingchun,
Wang Wenjuan,
Wei Yun
Publication year - 2021
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.202001243
Subject(s) - chemistry , chromatography , hesperidin , aqueous solution , high performance liquid chromatography , aqueous two phase system , organic chemistry , medicine , alternative medicine , pathology
The volatile oil of Mentha haplocalyx is widely used in medicine, food, and cosmetics. However, a large amount of its residue after steam extraction of volatile oil is abandoned, resulting in a waste of resources. The method of aqueous two‐phase flotation coupled with preparative high‐performance liquid chromatography was established for the separation and purification of nonvolatile active compounds from Mentha haplocalyx for the first time. The parameters of the two‐phase aqueous flotation were optimized. Under the optimal conditions including flotation solvent PEG 1000 aqueous solution (1:1, w/w), pH 5, (NH 4 ) 2 SO 4 concentration of 350 g/L in aqueous phase, N 2 flow rate of 20 mL/min, and flotation time of 20 min, the flotation efficiency of linarin, hesperidin, and didymin was 82.24, 76.38, and 89.33%, respectively. The linarin and hesperidin with the high purities of 95.8 and 97.2%, respectively, were obtained by using preparative high performance liquid chromatography. The neuroprotective effect of linarin against H 2 O 2 ‐induced oxidative stress in rat hippocampal neurons was investigated. The experimental result indicated that linarin could alleviate H 2 O 2 ‐induced oxidative stress. The work indicated that the combination of aqueous two‐phase flotation and preparative high performance liquid chromatography is a feasible and practical method for the purification of nonvolatile active substances from Mentha haplocalyx , which would provide a reference process for the comprehensive utilization of M. haplocalyx . Especially, linarin might be used as a good source of natural neuroprotectants.