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Hydrophilic carrageenan functionalized magnetic carbon‐based framework linked by silane coupling agent for the enrichment of N ‐glycopeptides from human saliva
Author(s) -
Jin Xueting,
Zhu Canhong,
Wu Jiani,
Yan Yinghua,
Ding ChuanFan,
Tang Keqi,
Zhang Di
Publication year - 2021
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.202001216
Subject(s) - glycopeptide , chemistry , mesoporous material , nanocomposite , selectivity , nanomaterials , silane , chemical engineering , monolith , substrate (aquarium) , copolymer , chromatography , materials science , organic chemistry , nanotechnology , polymer , biochemistry , oceanography , antibiotics , geology , engineering , catalysis
In this work, a magnetic graphene material coated with mesoporous silica was selected as the substrate, 3‐glycidoxypropyltrimethoxysilane and polyethyleneimine were sequentially bonded through chemical reactions, and then carrageenan was successfully introduced by electrostatic interaction; finally, hydrophilic nanocomposite material was prepared. Due to the large number of hydrophilic groups, and polyethyleneimine was connected by means of chemical bonds, this material exhibits good hydrophilicity and stability for glycopeptide enrichment. In the actual enrichment process, nanomaterial exhibits high selectivity (1:500), high sensitivity (2 fmol), and good repeatability (five cycles). In addition, the synthesized material also shows a good enrichment effect in the face of actual complex biological samples, which captured 40 N ‐glycopeptides from human saliva, indicating the application potential for enrichment of N ‐glycopeptides.