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Fast analysis of alkaloids from different parts of Mahonia bealei (Fort.) Carr. studied for their anti‐Alzheimer's activity using supercritical fluid chromatography
Author(s) -
Huang Yang,
Wang Tiejie,
Jiang Zhengjin
Publication year - 2021
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.202001079
Subject(s) - chromatography , supercritical fluid chromatography , chemistry , repeatability , supercritical fluid , detection limit , high performance liquid chromatography , gas chromatography , organic chemistry
In this study, a rapid and highly efficient method was developed for the separation of eight isoquinoline alkaloids using supercritical fluid chromatography. The separation conditions were carefully optimized including stationary phases, additives, backpressure, and temperature. Compared to high‐performance liquid chromatography, the use of supercritical fluid chromatography could provide a 13 times faster separation. Subsequently, the method was validated and applied for the determination of eight alkaloids from different parts of Mahonia bealei (Fort.) Carr. (stem, root, leaf, and seed). The results indicated a good repeatability with relative standard deviations for overall precisions lower than 3.2%. The limit of detection was between 0.4 and 2.3 μg/mL while limit of quantitation ranged from 1.5 to 7.5 μg/mL. Recovery ranged from 95.7 to 102.5% indicating a validity of recovery. The content of total eight alkaloids was the highest in stem (66.0 μg/g) and root (65.1 μg/g) compared to leaf or seed. Moreover, anti‐acetylcholinesterase activity for those extracts was evaluated by Ellman's colorimetric assay. As a result, the acetylcholinesterase inhibitory activity of the extracted samples was in the following decreasing order: stem > root > leaf or seed. In conclusion, the results indicated that supercritical fluid chromatography could be a useful tool for quality control of Mahonia bealei (Fort.) Carr. containing alkaloids as active compounds.