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Simultaneous quantification of arctigenin and its glucuronide conjugate in mouse plasma using ultra‐high performance liquid chromatography coupled to tandem mass spectrometry
Author(s) -
Suzuki Yosuke,
Sato Michiko,
Awazuhara Takuya,
Nukui Yusuke,
Yoshida Airi,
Terashima Tomoka,
Watanabe Keita,
Fujioka Rumi,
Tsuchihara Katsuya,
Kishino Satoshi,
Ohno Keiko
Publication year - 2021
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.202001078
Subject(s) - chemistry , glucuronide , chromatography , tandem mass spectrometry , liquid chromatography–mass spectrometry , mass spectrometry , metabolite , biochemistry
Arctigenin is a natural lignin and a main active component of Fructus arctii , the dried fruit of Arctium lappa . This compound was reported to have some biological activities such as anti‐inflammatory, antioxidant, antiviral, renoprotective, and antitumor effects. Arctigenin is mainly metabolized to arctigenin‐4′‐ O ‐glucuronide by UDP‐glucuronosyltransferase. In this study, a simultaneous quantification method was established and validated for measuring arctigenin and arctigenin‐4′‐ O ‐glucuronide in mouse plasma using ultra‐high performance liquid chromatography with tandem mass spectrometry. The assay fulfilled the requirements of the United States Food and Drug Administration guideline for assay validation, with a lower limit of quantification of 2.00 ng/mL for arctigenin and 50.0 ng/mL for arctigenin‐4′‐ O ‐glucuronide. The recovery rate and matrix effect ranged from 78.4 to 102.8% and 92.5 to 106.3%, respectively, for arctigenin, and 74.3 to 109.2% and 94.9 to 110.2% for arctigenin‐4′‐ O ‐glucuronide. The method was applied to the measurement of plasma concentrations of arctigenin and arctigenin‐4′‐ O ‐glucuronide in the plasma of mice after administration of arctigenin. All measured concentrations were within the calibration ranges. Our novel method may be useful to measure plasma arctigenin and arctigenin‐4′‐ O ‐glucuronide concentrations, and contribute to evaluate the pharmacokinetics of arctigenin and arctigenin‐4′‐ O ‐glucuronide in mice.