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Novel materials as capillary coatings for in‐tube solid‐phase microextraction for bioanalysis
Author(s) -
Grecco Caroline Fernandes,
Souza Israel Donizeti,
Queiroz Maria Eugênia Costa
Publication year - 2021
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.202001070
Subject(s) - solid phase microextraction , bioanalysis , chromatography , capillary action , sorbent , materials science , extraction (chemistry) , solid phase extraction , chemistry , mass spectrometry , adsorption , gas chromatography–mass spectrometry , organic chemistry , composite material
In‐tube solid‐phase microextraction with a capillary column as extraction device can be directly coupled with high‐performance liquid chromatography systems (HPLC). The in‐tube solid‐phase microextraction technique has been continuously developed since it was introduced in 1997. New couplings have also been evaluated on the basis of state‐of‐the‐art HPLC instruments. Different types of capillaries (wall‐coated open tubular, porous layer open tubular, sorbent‐packed, porous monolithic rods, or fiber‐packed) with selective stationary phases (monoliths, magnetic nanoparticles, conducting polymers, restricted access materials, ionic liquids, carbon, deep eutectic solvents, and hybrid materials) have been developed to boost in‐tube solid‐phase microextraction performance (sorption capacity and selectivity). This technique has been successfully applied to analyze biological samples (serum, plasma, whole blood, hair, urine, milk, skin, and saliva) for therapeutic drug monitoring, to study biomarkers, to detect illicit drugs, to conduct metabolomics studies, and to assess exposure to drugs. This review describes current advances in in‐tube solid‐phase microextraction extraction devices and their application in bioanalysis.