Premium
Rapid ultrasound‐assisted microextraction of atorvastatin in the sample of blood plasma by nickel metal organic modified with alumina nanoparticles
Author(s) -
Bahrani Sonia,
Ghaedi Mehrorang,
Asfaram Arash,
Mansoorkhani Mohammad Javad Khoshnood,
Javadian Hamedreza
Publication year - 2020
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.202000660
Subject(s) - nickel , atorvastatin , nanoparticle , chromatography , metal , chemistry , sample preparation , materials science , analytical chemistry (journal) , metallurgy , nanotechnology , medicine , endocrinology
In the present work, nickel‐1,4‐benzenedioxyacetic acid was synthesized as a rod‐like metal organic material and then modified with alumina nanoparticles to synthesize nickel metal organic modified‐Al 2 O 3 nanoparticles. The material was found as an efficient sorbent for the enrichment of atorvastatin in human blood plasma. After the extraction of the sample of plasma by ultrasound‐assisted dispersive solid phase extraction, high performance liquid chromatography‐ultraviolet was used to determine the quantitatively pre‐concentrated interest analyte. The conditions for optimum extraction were achieved by the optimization of the volume of eluent, dosage of the sorbent, and time of sonication. Solution pH of 7.0, 250 μL of ethanol, 45 mg of the sorbent, and 10 min of sonication time were the conditions for extracting the atorvastatin maximum recovery of higher than 97.0%. By using desirability function for the optimization of the process, the present method showed a response that was linear ranging from 0.2 to 800 ng/mL with regression coefficient of 0.999 in the plasma of human blood with a satisfactory detection limit of 0.05 ng/mL, while the precision of interday for the current method was found to be <5%. It can be concluded that dispersive solid phase extraction method is effective for the extraction of atorvastatin from human plasma samples (97.4‐102%) due to its easy operation, simplicity, repeatability, and reliability.